ฐานข้อมูลอันตรกิริยาระหว่างสมุนไพรกับยาแผนปัจจุบัน

Part Used : ไม่ระบุ

Activity : CYP3A4 INHIBITION

Solvent/Active Compound : Diosgenin and dioscin

Type of experiment : in vitro

Type of animal : -

Type of study : -

N(Total) : -

N(Treatment) : -

Sex : -

Age : -

Route : Non-specified

Dose/Conc.(herb) : *

Duration : *

Type of interaction : Pharmacokinetics

Interaction with drug : -

Dose/Conc.(drug) : -

Result : Positive

Remark : Result: Diosgenin and dioscin inhibited CYP3A4 with IC50 values of 17 and 33 micro molar, respectively, while other CYP enzymes were not affected.

Note : *Dose/Duration: The assay was performed in a total volume of 200 micro liters using 96-well microtiter plates. Test compounds (50-0.2 micro molar) or postive control (5.0.02 micro molar), cofactors mix, and G-6-PDH were added to the wells and pre incubated for 10 min at 37 degrees celsius, and the plates were read to account for any fluorescence caused by the test compounds (0-min readouts, both diosgenin and dioscin had no measurable auto fluorescence). Reaction was initiated by adding the enzyme substrate mixtre and incubated for 30 min. Reaction was stopped by adding ice cold acetonitrile/0.5M Trisbase (80:20).

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