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Part Used : ไม่ระบุActivity : CYP2D6 INHIBITIONSolvent/Active Compound : Diosgenin and dioscinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : Non-specifiedDose/Conc.(herb) : *Duration : *Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark :Note : *Dose/Duration: The assay was performed in a total volume of 200 micro liters using 96-well microtiter plates. Test compounds (50-0.2 micro molar) or postive control (5.0.02 micro molar), cofactors mix, and G-6-PDH were added to the wells and pre incubated for 10 min at 37 degrees celsius, and the plates were read to account for any fluorescence caused by the test compounds (0-min readouts, both diosgenin and dioscin had no measurable auto fluorescence). Reaction was initiated by adding the enzyme substrate mixtre and incubated for 30 min. Reaction was stopped by adding ice cold acetonitrile/0.5M Trisbase (80:20).
Part Used : เหง้าActivity : CYP2D6 INHIBITIONSolvent/Active Compound : ethanolType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The selective inhibitor quinidine showed the most potent inhibitory activity on CYP2D6 activity with mean IC50 of 0.97 microgram/mL. DM, DL and PI showed the highest inhibitory activities (mean IC50 2.93-9.57 microgram/mL). The potency of inhibitory activity of DM was comparable to that of quinidine (p > 0.05). PC, GM and ZO exhibited moderate potencies (mean IC50 23.40-31.32 microgram/mL), and MF and AL exhibited relatively low potencies (mean IC50 195.83 and 313.51 microgram/mL, respectively). (substrate: dextromethorphan)Note : Piper chaba (PC), Dioscroea membranacea (DI), Dracaenal oureiri (DG), Atractylodes lancea (AL), Plumbago indica (PI), Zingiber officinale (ZO), Myristica fragrans (MF), Garcinia mangostana (GM).
