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Part Used : ไม่ระบุActivity : CYP1A2 INHIBITIONSolvent/Active Compound : Diosgenin and dioscinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : Non-specifiedDose/Conc.(herb) : *Duration : *Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark :Note : *Dose/Duration: The assay was performed in a total volume of 200 micro liters using 96-well microtiter plates. Test compounds (50-0.2 micro molar) or postive control (5.0.02 micro molar), cofactors mix, and G-6-PDH were added to the wells and pre incubated for 10 min at 37 degrees celsius, and the plates were read to account for any fluorescence caused by the test compounds (0-min readouts, both diosgenin and dioscin had no measurable auto fluorescence). Reaction was initiated by adding the enzyme substrate mixtre and incubated for 30 min. Reaction was stopped by adding ice cold acetonitrile/0.5M Trisbase (80:20).
Part Used : เหง้าActivity : CYP1A2 INHIBITIONSolvent/Active Compound : ethanolType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The selective inhibitor of CYP1A2 alpha-naphthoflavone exhibited the most potent inhibitory activity on enzyme activity with mean IC50 of 0.01 microgram/mL. PC and AL exhibited most potent activity with IC50 values in a similar range of that of the alpha-naphthoflavone (p > 0.05). DM, DL, GM and ZO exhibited moderate potencies (IC50 1.04-9.87 microgram/mL), whereas PI and MF exhibited relatively low potencies (IC50 12.95 and 22.05 microgram/mL, respectively) (Substrate=phenacetin)Note : Piper chaba (PC), Dioscroea membranacea (DI), Dracaenal oureiri (DG), Atractylodes lancea (AL), Plumbago indica (PI), Zingiber officinale (ZO), Myristica fragrans (MF), Garcinia mangostana (GM).
