Synonym |
Thai / English name |
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : Aqueous extractType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were 288.8 microgram/mL.Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : methanol extractType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were >500 microgram/mL.Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : dichloromethane extractType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were 133.2 microgram/mL.Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : Petroleum ether extractType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were 142.1 microgram/mL.Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : Rosmarinic acidType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were 505.9 microgram/mL. (1469.3 microgram)Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : sinensetinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : IC50 values for inhibition of CYP2D6 were >200 microgram/mL. (>537.1 microgram)Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ทั้งต้นActivity : CYP2D6 INHIBITIONSolvent/Active Compound : eupatorinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : IC50 values for inhibition of CYP2D6 were 3.8 microgram/mL. (11.0 micromolar) and a Ki value of 3.5 microgram/mL (10.2 micromolar) with uncompetitive inhibition mode.Note : - Orthosiphon stamineus (OS) components with IC50 values less than 100 microgram/mL (100 micromolar for active constituents) were subsequently determined for Ki (inhibition constant) values and inhibition modes. - In order to determine the modulatory effects of OS components on CYP activities, the enzyme assays were carried out in the presence and absence of OS extracts or active constituents. Metabolite (4-hydroxytolutamide, dextrophan and 6beta-hydroxytestosterone) formations were estimated using HPLC system. For each inhibition study, preliminary experiments were carried out by incubating a single enzyme specific substrate concentration around its Km with a range of OS extract and active constiuent concentrations to determine IC50 values (concentration on inhibitor causing 50% inhibition of enzyme activity).
Part Used : ไม่ระบุActivity : CYP2D6 INHIBITIONSolvent/Active Compound : ethanolType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The ethanolic extract of Orthosiphon aristatus inhibited CYP2D6 activity with IC50 value 31.0+/-19.5 microgram/ml.Note : Testosterone 6beta-hydroxylase and dextromethorphan O-demethylase were used as maker of CYP3A4 and CYP2D6 activity, respectively.
Part Used : ไม่ระบุActivity : CYP2D6 INHIBITIONSolvent/Active Compound : aqueousType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The aqueous extract of Orthosiphon aristatus inhibited CYP2D6 activity with IC50 value 406.7+/-141.9 microgram/ml.Note : Testosterone 6beta-hydroxylase and dextromethorphan O-demethylase were used as maker of CYP3A4 and CYP2D6 activity, respectively.