Synonym |
Thai / English name |
Part Used : ใบActivity : CYP1A2 INHIBITIONSolvent/Active Compound : 100% methanolType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark :Note : - Dried, plant samples (~10 g) were extracted in 100 mL of 100% methanol (MeOH) evernight. - Plant extracts were screened for the ability to induce CYP1A2 and CYP3A4. 1A2DRE (human XRECYP1A2-luciferase promoter) and DPX2 (human PXRCYP3A4-luciferase promoter) in hepatoma cell lines. Criteria for "positive" plant samples was set at ~40% of the response seen for the prototype inducers, i.e., > 40-fold in luciferase activity for CYP1A2 and > 3.5-fold increase for CYP3A4. - Plant extracts were screened for inhibitiory activity towards CYP1A2, CYP3A4, and CYP2D6 using enzyme-selective model substrates in human liver microsomes. Methoxyresorufin (MR) was used to assess CYP1A2 activity, 7-benzyloxyquinoline (7-BQ) was used to assess CYP3A4 activity and 7-methoxy-4-(aminomethyl)-coumarin (MAMC) was used to assess CYP2D6 activity. Extracts showing >50% inhibition at half-saturating and/or saturating substrate concentrations were deemed inhibitory.
Part Used : น้ำจากผลActivity : CYP1A2 INHIBITIONSolvent/Active Compound : -Type of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : 7.81-250 mg/mlDuration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : Type of experiment: mouse hepatic microsomes Results: The guava exhibited reductions of the EROD, MROD, and ENDM activities.Note : Four enzymatic reactions: ethoxyresorufin O-deethylation (EROD) for CYP1A1, ethoxyresorufin O-demethylation (MROD) for CYP1A2, aniline hydroxylation (ANH) for CYP2E1, and erythromycin N-demethylation (ENDM) for CYP3A11.
Part Used : ใบActivity : CYP1A2 INHIBITIONSolvent/Active Compound : guava leaves extractType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The extract of guava leaves (GvEx) inhibited CYP2C8, CYP2C9 and CYP3A4 (IC50: 18.16 micrograms/mL, 28.00 micrograms/mL, 19.92 micrograms/mL, respectively). In contrast, quercetin contained at 3.5% in the GvEx had a more than tenfold higher inhibitory activity in these P 450 tests (IC50: 0.59 micrograms/mL, 1.62 micrograms/mL, 1.50 micrograms/mL). The GvEx inhibited CYP2D6, CYP2C19, CYP2B6 and CYP1A2 several fold weaker than quercetin.Note : Data in complete
Part Used : ใบActivity : CYP1A2 INHIBITIONSolvent/Active Compound : guava teaType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : NegativeRemark : Bansoreicha (a guava tea product) did not inhibit these P 450 enzymes.Note : Data in complete