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| Thai / English name |
Part Used : เมล็ดActivity : CYP3A4 INHIBITIONSolvent/Active Compound : NeferineType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : 5 microlitersDuration : 10 minutesType of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : -Result : PositiveRemark : The IC50 values of neferine on CYP3A4 was 25.1+/-3.5 micromolar (15.7+/-2.1 micrograms/mL).
Part Used : ใบActivity : CYP3A4 INHIBITIONSolvent/Active Compound : 80% ethanolType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : 10, 50, 100, 200 and 400 micrograms/mL.Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The inhibitory effects of LAE on CYP3A4 isoenzyme wiht IC50 values 98.01 micrograms/mL.Note : - Their Lineweaver-Burk plots and Dixon plots showed that NF, N-NF, and HMA competitively inhibited CYP2D6 activity with Ki values of 1.88, 2.34 and 1.56 micromolars, respectively - Conclusion, lotus leaves exert a potent inhibitory effect on CYP2D6 isoenzyme. - Abbreviation LAE lotus leaf alcoholic extract AF the alkaloid fraction FF the flavonoid fraction NF nuciferine N-NF N-nornuciferine HMA 2-hydroxy-1-methoxyaporphine
Part Used : ใบActivity : CYP3A4 INHIBITIONSolvent/Active Compound : alkaloid fraction (AF)Type of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : 0.5-250 micrograms/mL.Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The inhibitory effects of AF on CYP3A4 isoenzyme wiht IC50 values 44.87 micrograms/mL.Note : - Their Lineweaver-Burk plots and Dixon plots showed that NF, N-NF, and HMA competitively inhibited CYP2D6 activity with Ki values of 1.88, 2.34 and 1.56 micromolars, respectively - Conclusion, lotus leaves exert a potent inhibitory effect on CYP2D6 isoenzyme. - Abbreviation LAE lotus leaf alcoholic extract AF the alkaloid fraction FF the flavonoid fraction NF nuciferine N-NF N-nornuciferine HMA 2-hydroxy-1-methoxyaporphine
Part Used : ใบActivity : CYP3A4 INHIBITIONSolvent/Active Compound : flavonoid fraction (FF)Type of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : 2-250 micrograms/mL.Duration : -Type of interaction : PharmacokineticsInteraction with drug : -Dose/Conc.(drug) : -Result : PositiveRemark : The inhibitory effects of FF on CYP3A4 isoenzyme wiht IC50 values 97.50 micrograms/mL.Note : - Their Lineweaver-Burk plots and Dixon plots showed that NF, N-NF, and HMA competitively inhibited CYP2D6 activity with Ki values of 1.88, 2.34 and 1.56 micromolars, respectively - Conclusion, lotus leaves exert a potent inhibitory effect on CYP2D6 isoenzyme. - Abbreviation LAE lotus leaf alcoholic extract AF the alkaloid fraction FF the flavonoid fraction NF nuciferine N-NF N-nornuciferine HMA 2-hydroxy-1-methoxyaporphine
Part Used : เมล็ดActivity : CYP3A4 INHIBITIONSolvent/Active Compound : neferineType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : -Duration : -Type of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : -Result : PositiveRemark : Result: Neferine showed a significant mechanism-based inhibition of CYP3A4. Neferine displayed Pi bonds with the heme group of CYP3A4, which may produce a mechanism-based inhibition by the heme group pathway. Combined with the CYP3A4 substrate, it is reasonable to believe that Nef has a mixed-type inhibition or positive cooperativity of CYP3A4 mediated testosterone metabolism.Note : Phenacetin, dextromethorphan and testosterone, respectively, were used as CYP1A2, CYP2D6 and CYP3A4 substrates, and their metabolites were determined by validated HPLC methodologies.
