Part Used : เมล็ด
Activity : CYP1A2 INHIBITION
Solvent/Active Compound : neferine
Type of experiment : in vitro
Type of animal : -
N(Total) : -
N(Treatment) : -
Route : -
Dose/Conc.(herb) : -
Duration : -
Type of interaction : Pharmacokinetics
Interaction with drug : Phenacetin*/Acetophenetidin
Dose/Conc.(drug) : -
Remark : Result: Neferine showed a nonmechanistic inhibition of CYP1A2. Neferine inhibits CYP1A2 activity only to a minor extent, and in this study, demonstrates a non-time-dependent increase in the inhibition of CYP1A2, meaning that Nef did not fulfil the criteria as a mechanism-based inhibitor of CYP1A2. Consequently, from this study, it can be assumed the Neferine displays a weak or negligible inhibiton of CYP1A2 and CYP2D6 in vitro.
Note : Phenacetin, dextromethorphan and testosterone, respectively, were used as CYP1A2, CYP2D6 and CYP3A4 substrates, and their metabolites were determined by validated HPLC methodologies.