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Part Used : เปลือกต้น

Activity : CYP1A2 INHIBITION

Solvent/Active Compound : 100% methanol

Type of experiment : in vitro

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N(Treatment) : -

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Type of interaction : Pharmacokinetics

Interaction with drug : -

Dose/Conc.(drug) : -

Result : Positive

Remark : Medicinal plant induction of CYP3A4 was <4.0 fold.

Note : - Dried, plant samples (~10 g) were extracted in 100 mL of 100% methanol (MeOH) evernight. - Plant extracts were screened for the ability to induce CYP1A2 and CYP3A4. 1A2DRE (human XRECYP1A2-luciferase promoter) and DPX2 (human PXRCYP3A4-luciferase promoter) in hepatoma cell lines. Criteria for "positive" plant samples was set at ~40% of the response seen for the prototype inducers, i.e., > 40-fold in luciferase activity for CYP1A2 and > 3.5-fold increase for CYP3A4. - Plant extracts were screened for inhibitiory activity towards CYP1A2, CYP3A4, and CYP2D6 using enzyme-selective model substrates in human liver microsomes. Methoxyresorufin (MR) was used to assess CYP1A2 activity, 7-benzyloxyquinoline (7-BQ) was used to assess CYP3A4 activity and 7-methoxy-4-(aminomethyl)-coumarin (MAMC) was used to assess CYP2D6 activity. Extracts showing >50% inhibition at half-saturating and/or saturating substrate concentrations were deemed inhibitory.

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