Part Used : ไม่ระบุ
Activity : CYP3A INHIBITION
Solvent/Active Compound : 70% ethanol
Type of experiment : in vivo
Type of animal : rabbit
N(Total) : -
N(Treatment) : -
Route : Oral administration
Dose/Conc.(herb) : 500 mg/kg
Duration : 7 days
Type of interaction : Pharmacokinetics
Interaction with drug : Midazolam*/Versed/Dormicum
Dose/Conc.(drug) : 10 mg/kg
Remark : Results: Pretreatment with P. amarus significantly increased the mean maximum concentration (Cmax), time to reach maximum concentration (Tmax), area under curve (AUCo-8), and elimination half-life (T1/2) (2.9-, 1.6-, 2.8-, and 1.4-fold, respectively) compared with control group receiving a single oral dose of midazolam. The results suggest that P. amarus extract inhibits midazolam metabolism in rabbits probably by inhibition of CYP3A4 which is the enzyme responsible for midazolam metabolism.
Note : The animals were orally administered with 500 mg/kg P. amarus extract once a day for 7 days and the last dose in morning on day 8, one hour before midazolam administration. Blood samples were taken marginal ear vein through a venous catheter before administration of midazolam and at 0.25, 0.5, 0.75, 1, 2, 3, 4, 6 and 8 h post dose.
