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Part Used : น้ำจากผลActivity : CYP3A4 INHIBITIONSolvent/Active Compound : Methanol elutes of ethyl acetate extracts of orange juiceType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : The reaction mixture, consisting of 1.3 mM NADP, 3.3 mM glucose 6-phsophate, 0.4 U/ml of glucose-6-phosphate dehydrogenase, 3.3 mM magnesium chloride, and 0.2 mM testosterone in 100 mM potassium phosphate buffer (pH 7.4), was preincubated for 5 min at 37 degrees celsius in the presence of ethyl acetate extracts of orange juice. The reaction was started by the addition of 1.25 pmol of P450, in the case of human CYP3A4, and 0.05 mg of protein, in the case of human liver microsomes. After incubation for 15 min at 37 degrees celsius, the reaction was stopped by the addition of methylene chloride.Duration : 15 min.Type of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : 0.2 nM testosteroneResult : NegativeRemark : Result: No significant inhibitory effect on testosterone 6beta-hydroxylation (endogenous substrate of cytochrome P450 3A4) was observed in any of the methanol eluates (0 - 100% methanol eluates).
Part Used : น้ำจากผลActivity : CYP3A4 INHIBITIONSolvent/Active Compound : 3,3',4',5,6,7,8-heptamethoxyflavone (HMF)Type of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : The reaction mixture, consisting of 1.3 mM NADP, 3.3 mM glucose 6-phosphate, 0.4 U/ml of glucose-6-phosphate dehydrogenase, 3.3 mM magnesium chloride, and 0.2 mM testosterone in 100 mM potassium phosphate buffer (pH 7.4), was preincubated for 5 min at 37 degrees celsius in the presence of HMF 0.1, 1, 10, 100 microM. The reaction was started by the addition of 1.25 pmol of P450, in the case of human CYP3A4, and 0.05 mg of protein, in the case of human liver microsomes. After incubation for 15 min at 37 degrees celsius, the reaction was stopped by the addition of methylene chloride.Duration : 15 min.Type of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : 0.2 mM testosteroneResult : NegativeRemark : Result: Neither compound markedly inhibited 6beta-hydroxylation of testosterone by human liver microsomes or recombinant human CYP3A4.
Part Used : น้ำจากผลActivity : CYP3A4 INHIBITIONSolvent/Active Compound : tangeretinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : The reaction mixture, consisting of 1.3 mM NADP, 3.3 mM glucose 6-phosphate, 0.4 U/ml of glucose-6-phosphate dehydrogenase, 3.3 mM magnesium chloride, and 0.2 mM testosterone in 100 mM potassium phosphate buffer (pH 7.4), was preincubated for 5 min at 37 degrees celsius in the presence of tangeretin 0.1, 1, 10, 100 microM. The reaction was started by the addition of 1.25 pmol of P450, in the case of human CYP3A4, and 0.05 mg of protein, in the case of human liver microsomes. After incubation for 15 min at 37 degrees celsius, the reaction was stopped by the addition of methylene chloride.Duration : 15 min.Type of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : 0.2 mM testosteroneResult : NegativeRemark : Result: Neither compound markedly inhibited 6beta-hydroxylation of testosterone by human liver microsomes or recombinant human CYP3A4.
Part Used : น้ำจากผลActivity : CYP3A4 INHIBITIONSolvent/Active Compound : nobiletinType of experiment : in vitroType of animal : -Type of study : -N(Total) : -N(Treatment) : -Sex : -Age : -Route : -Dose/Conc.(herb) : The reaction mixture, consisting of 1.3 mM NADP, 3.3 mM glucose 6-phosphate, 0.4 U/ml of glucose-6-phosphate dehydrogenase, 3.3 mM magnesium chloride, and 0.2 mM testosterone in 100 mM potassium phosphate buffer (pH 7.4), was preincubated for 5 min at 37 degrees celsius in the presence of nobiletin 0.1, 1, 10, 100 microM. The reaction was started by the addition of 1.25 pmol of P450, in the case of human CYP3A4, and 0.05 mg of protein, in the case of human liver microsomes. After incubation for 15 min at 37 degrees celsius, the reaction was stopped by the addition of methylene chloride.Duration : 15 min.Type of interaction : PharmacokineticsInteraction with drug : TestosteroneDose/Conc.(drug) : 0.2 mM testosteroneResult : NegativeRemark : Result: Neither compound markedly inhibited 6beta-hydroxylation of testosterone by human liver microsomes or recombinant human CYP3A4.
