Part Used : น้ำมันจากเมล็ด
Activity : GLUTATHIONE DEPLETION
Solvent/Active Compound : corn oil (linoleic acid)
Type of experiment : in vivo
Type of animal : rat
N(Total) : -
N(Treatment) : -
Route : Oral administration
Dose/Conc.(herb) : Feeding diets with 15% corn oil + 6% protein* (Group 1) for six weeks. After the feeding period, liver injury was induced by injecting either with 600 mg/kg body weight acetaminophen suspended in gum arabic-based vehicle.
Duration : 6 weeks
Type of interaction : Pharmacodynamics
Interaction with drug : Paracetamol*/Acetaminophen/APAP/N-acetyl-p-aminophenol
Dose/Conc.(drug) : Acetaminophen 600 mg/kg body weight
Remark : Result: glutathione (GSH) level was significantly lower in only group fed 15% corn oil with 6 g/100 g protein among acetaminophen-treated groups, activities of Glutamate-pyruvate transaminase (GPT) and glutamate-oxaloacetate transaminase (GOT) were significantly elevated in all groups except the one fed beef tallow with 20 g/100 g protein, suggesting low protein might exacerbate drug-induced hepatotoxicity.
Note : The feeding regimens changed the ratio of 18:2n-6 to oleic acid (18:1n-9) in total liver lipids approximately five-fold, and produced modest changes in arachidonic acid (20:4n-6). Conclusion: Diets with high 18:2n-6 promote acetaminophen-induced liver injury compared to diets with more saturated fatty acids (SFA). In addition, protein restriction appeared to exacerbate the liver injury.
*Dose: protein = casein:lactoalbumin (1:1)
